glycosidases in human blood plasma and urine. molecular forms and activity levels

the isoe lect r i c f ocusing p a t terns and ph activity cur ves of f i ve glycosidas e e nzymes i n blood plasma and ur i ne were s t ud i e d . this s tudy demons trated the presence o f a sing le molecular f onn in a -glucosidase and of two mole cul a r fonns of the enzymes h-a ce t yl-b-d-gluco sarni nidase, na c e t y l -•p-d-g a l a c t o s amin i ua s e and s-glucuronidase i n both plasma and urine . b-galactosidase existed as a s i ng le major molecular f orm i n p l asma and a t l e a st t wo molecular forms in urine. spe cific activity of each~ le n zyme was determined in p lasma and urine the validity o f taking the ratio between two e n zvrne activities as an index for scr een .l.ng of fr ene t ic di s e a s e is discussed.